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CO2 Laser-assisted Sclerectomy Surgery for Open-angle Glaucoma

Published Online: February 8th 2012 European Ophthalmic Review, 2012;6(1):12-6 DOI: http://doi.org/10.17925/EOR.2012.06.01.12
Authors: Noa Geffen, Yokrat Ton, Gonzalo Muñoz, Cesare Mariotti, Ehud I Assia
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Abstract:
Overview

Purpose: To evaluate the safety and performance of CO2 laser-assisted sclerectomy surgery (CLASS). Materials and Methods: CLASS using the IOPtiMate™ system was performed in experimental models and in patients with primary and pseudoexfoliative open-angle glaucoma. CO2 laser was used to achieve deep scleral ablation. Percolation and perforation rates were recorded. Histopathological analysis was performed on laboratory models, complications were recorded and postoperative intraocular pressure (IOP) was measured. Results: Deep scleral ablation and aqueous percolation were repeatedly achieved. Histology disclosed deep scleral craters with a thin intact sclero-corneal tissue at the ablation area with mild, transient and limited thermal damage. Thirty of 37 patients with glaucoma completed 12 months of follow-up. The baseline IOP of 26.3 ± 7.8 mmHg (mean ± SD) dropped to 14.4 ± 3.4 mmHg and 14.3 ± 3.1 mmHg at six and 12 months, respectively (p<0.001). Complications were mild and transitory with no sequelae. Conclusions: CLASS using the IOPtiMate system is a safe and efficacious procedure for achieving effective fluid percolation.

Acknowledgements: Study group (alphabetic order): S Anisimova, EI Assia, M Belkin, F Gil Carrasco, E Dahan, J Degani, N Geffen, O Geyer, D Kidron, G Kleinman, S Kurtz, C Mariotti, G Muñoz, S Naveen, M Sherwood, R Thomas, Y Ton, M Turati, M Zalish.

Keywords

Glaucoma, filtration, non-penetrating, deep sclerectomy, laser surgery

Article:

Conventional trabeculectomy has so far remained the gold standard for glaucoma surgery, despite its potential vision-threatening complications.1 Efforts are being made to develop a new surgical approach to overcome the limited success rate and safety issues of the traditional trabeculectomy. The pioneering work of Krasnov2 and the various modifications that succeeded it2–10 led to the development of a filtration procedure known as non-penetrating deep sclerectomy (NPDS). NPDS is known to have a higher safety profile compared with trabeculectomy but one of the main drawbacks of the procedure is its technical difficulty.11 A frequent complication of manual NPDS12 is inadvertent perforation into the anterior chamber (AC), necessitating conversion to a penetrating filtration procedure. Insufficient tissue dissection, which is another common problem related to technical difficulties, prevents effective fluid percolation and intraocular pressure (IOP) reduction.13
Albert Einstein established the theoretic foundations for the laser in 191714 and the concept was further developed over the years. There is a great interest in using lasers for glaucoma treatment. Theoretically, laser-assisted filtration surgery offers the potential advantage of improved accuracy, repeatability and safety.15 The main disadvantage is the potential collateral damage induced by scattered energy at the filtration site, which may lead to enhanced scarring, and may be detrimental to the long-term success of the filtering procedure.16 Using a laser with high water absorbance and low light scattering may reduce the extent of collateral thermal damage and improve the long-term surgical success.17 CO2 laser was one of the earliest gas lasers to be developed.18 CO2 laser characteristics include photoablation of dry tissue and coagulation of bleeding vessels, and effective absorption of laser energy by any water or aqueous present, even if only in a minimal amount. Assia and colleagues suggested using the CO2 laser for the treatment of patients with open-angle glaucoma and called the filtration procedure CO2 laser-assisted sclerectomy surgery (CLASS).11,17 The procedure includes manual creation of a superficial scleral flap followed by progressive CO2 laser ablation of the scleral tissue. The ablation ceases when aqueous percolation is achieved as fluid absorbs the laser energy and prevents penetration through the remaining thinned scleral wall.
Preliminary studies of CLASS in animals and in human cadaver eyes,19 and pilot clinical studies of CLASS with the first model of the CO2 laser system (OT-133),11 have demonstrated its efficacy in achieving fluid percolation with significant IOP reduction and a low perforation rate. CLASS using the OT-133 was found to be a safe and relatively simple procedure, but there were also several drawbacks, such as excessive charring and tissue coagulation around the treated area. Clinically, it was evident that failure had occurred in some cases because of early adhesions and synechiae formation. A second-generation system was then developed, IOPtiMate™ (IOPtima Ltd, Ramat-Gan, Israel) using a higher power laser and an advanced beam manipulator and scanner. Increasing the laser power and decreasing the beam dwell time resulted in increased control of the ablation process while decreasing residual momentary heating and tissue coagulation.
The objectives of the following trials were to evaluate the safety and performance of the IOPtiMate system in performing CLASS in a preclinical trial followed by a clinical trial.

Materials and Methods
Surgical Procedure

Following fornix-based superior peritomy and removal of the tenon capsule, a half-thickness rectangular limbal-based 5 mm x 5 mm scleral flap was dissected at the limbus into the clear cornea. A red laser (HeNe, ~200 μm spot size) aiming beam was used to mark the scanning area boundaries (Figure 1). Scleral ablation was performed with the CO2 laser system (40C, Lumenis, Yokneam, Israel). The laser beam was focused and applied on the treated eye using a beam-manipulating system attached to an ophthalmic microscope. The IOPtiMate system consists of a scanner, used to control the shape, size and scanning parameters of the focused laser beam, a micromanipulator that attaches the system to the ophthalmic microscope and accurately positions the laser-scanned pattern on the desired ablation area, and a focusing assembly. Operation of the scanner is regulated by a control unit and the operating parameters are presented on a control panel. Scan dimensions (width and length) could be changed within the range of 1–4 mm. Initially, a wide scan area (e.g. 2.4 x 2.0 mm) was used to repeatedly remove layers of sclera until the percolation zone could be readily identified by the clear signs of percolation. The ablation area was then reduced and adjusted to target Schlemm’s canal in human eyes, or the plexus area in pig and rabbit eyes. The CO2 laser was repeatedly applied with intervals of two to three seconds between applications to allow percolation to take place and be detected. Residual charred tissue was wiped away with a BSS damp Weck-Cel sponge and ablation was continued until sufficient percolation was achieved along a region of at least 3 mm in length (Figure 1). The scleral flap was repositioned and secured with two interrupted 10-0 nylon sutures.
In the living eyes, a high-molecular-weight ophthalmic viscosurgical device (Healon® 5, Abbott Medical Optics, Santa Ana, California, USA) was applied beneath the flap, the conjunctiva was adequately secured with 2-4 10-0 nylon buried sutures, and the eye was patched with antibiotic and steroid ointments. Living rabbits were anaesthetised using intramuscular ketamine (35 mg/kg) and xylazine (5 mg/kg) whereas patients with glaucoma were operated under subconjunctival anaesthesia with 2 % lidocaine without epinephrine. All living eyes were treated postoperatively with topical steroids and antibiotic drops.
Mitomycin C (MMC) was not used in the preclinical phase, whereas the application of MMC and its concentration were left to the surgeon’s discretion in the clinical trial.

Preclinical Phase

The preclinical trial, as previously described by our group,20 included an ex vivo (enucleated porcine and human cadaver eyes) and an in vivo model (12 New Zealand white healthy male rabbits). Aqueous percolation sufficiency and rate of perforations during scleral ablation were assessed. Histological examination included evaluation of the scleral crater formed at the ablated sclera, assessment of the dimensions and integrity of the remaining trabeculo-Descemet’s membrane, and grading of the mechanical and thermal damage to treated and adjacent tissues. Assessment of in vivo results focused on the inflammatory reaction, thermal damage at the ablated site, and the healing process.

Clinical Phase

This was a prospective, non-randomised, non-comparative, multinational, multicentre clinical study.21 The trials were carried out in Mexico City (Drs Carrasco and Turati), in Madanapelee, India (Dr’s Thomas and Naveen), in Moscow, Russia (Dr Anisimova), in Ancona, Italy (Dr C Mariotti) and in Valencia, Spain (Dr G Muñoz).
Eligible participants were men and women, aged 18 or older, phakic or pseudophakic, with primary open-angle glaucoma (POAG) or pseudoexfoliative glaucoma (PEXFG). The clinical diagnosis was based on findings of glaucomatous optic neuropathy and of reliable and reproducible evidence of visual field defects typical of glaucoma. Primary filtration surgery was indicated in each participant, all of whom were on maximal tolerated hypotensive medications and had an IOP in the study eye of 18 mmHg or higher, as measured with a Goldmann applanation tonometer during three consecutive visits over a 90-day period prior to enrolment. The inclusion and exclusion criteria, follow-up schedule and assessments performed at each study visit have been fully described by our group.21 Follow-up duration was 12 months. Intraoperative and postoperative complications were classified according to severity and their relationship to the studied device. Also recorded was the incidence of intraoperative macro-perforations, defined as perforations accompanied by iris prolapse and/or AC shallowing.
‘Complete success’ was defined as 5≤ IOP≤ 18 mmHg measured at the six-month visit and 12-month endpoint, and IOP reduction ≥20 % compared with baseline without additional hypotensive medications or repeat filtration surgery. The same finding, but also including patients who required hypotensive medications postoperatively, was defined as ‘qualified success’. Failure was defined as an IOP value <5 mmHg and >18 mmHg, IOP reduction of less than 20 % compared with baseline, severe loss of vision, or the need to undergo additional glaucoma surgery. Goniopuncture and needling were not considered to be failures or adverse events as both are commonly used as accepted and common postoperative interventions that are required to maintain or augment the operative results of glaucoma surgeries.22,23 Goniopuncture and needling procedures were indicated when IOP was above the target pressure of the individual patient as determined by the surgeon. Decisions about which procedure to perform and the timing of performance were left to the surgeon’s discretion. The number of hypotensive medications being used at the six- and 12-month visits was compared with the baseline situation.

Statistical Methods

Student’s t-test was used to compare postoperative and baseline IOP in the in vivo laboratory model. Statistical analysis of the clinical trial included summation of continuous variables, including the mean, median, standard errors, and minimum and maximum values. Categorical variables were derived from frequency counts and percentages.
The 95 % confidence intervals (CIs) were calculated for the mean IOP measurements and for the success rates at six-month follow-up and the 12-month endpoint. A paired t-test was used to determine the significance of the changes in IOP. All tests applied were two-tailed, and a p value of 0.05 or less was considered significant. Data were analysed using the SAS® software (SAS Institute, Cary, North Carolina, USA).

Results
Preclinical Phase

As shown in Table 1, aqueous percolation was repeatedly achieved without the occurrence of macro-perforations during the procedures. Tissue histology demonstrated a funnel-shaped scleral crater with a thin residual corneo-scleral layer in the deeper aspect (Figure 2). The lateral walls of the crater created in the porcine eyes and in human cadaver eyes showed a thin layer of thermal damage. However, at the bottom of the crater over the exposed plexus/Schlemm’s canal where percolation takes place, thermal damage was minimal or absent. There was no clinical or histological evidence of thermal damage to adjacent tissues such as the iris, ciliary body or cornea.
The mean baseline IOP in rabbits eyes was 16.6 ± 2.8 mmHg (mean ± SD) dropped by 6.3 ± 3.9 mmHg (n=16, p<0.0001) on the first postoperative day and remained 2 mmHg below baseline up to Day 9. There were no cases of persistent hypotony or ocular hypertension. In the rabbits sacrificed immediately after surgery (seven eyes) histological examination disclosed a deep crater formed in the scleral wall down to the trabeculo-Descemet’s membrane filled with blood, plasma, fibrin and neutrophils. Thermal damage to tissues at the crater walls and mild damage at the crater floor were seen in four cases. In the rabbits sacrificed 10, 15 and 21 days postoperatively the crater was filled with loose connective tissue. A fine basophilic line delineated the tissue that had sustained thermal damage. No inflammatory cells were seen in the AC or vitreous cavity.

Clinical Phase

The first 37 patients (from Russia, India and Mexico) have completed one year of follow-up and their results are presented. Twenty-five patients were later recruited in two additional sites (Spain and Italy). The one-year data are being collected and will be published once analysed.
Twenty-eight patients (75.7 %) with POAG and nine (24.3 %) with PEXFG who met the inclusion/exclusion criteria were enrolled in the study.21 Five patients were excluded from the performance analysis.21 Twenty-one (56.8 %) were men and the mean age was 63.4 ± 11.8 years. MMC was used in 25 patients (67.56 %).

Safety Analysis

Safety analysis revealed no device malfunctions and there were no device-related macro-perforations. Four cases of micro-perforation were recorded. The AC remained deep and stable in all cases. Postoperative procedure-related complications occurred in eight patients (21.6 %): peripheral anterior synechiae (2), choroidal detachment (2), wound leak (2), dellen (1) and hyphema (1). All but one complication were graded as mild and resolved spontaneously or with conservative treatment within month after surgery, and none was attributed specifically to the laser treatment. One patient developed choroidal detachment one week postoperatively and was treated using drainage with complete recovery.

Performance Analysis

The preoperative IOP of 26.3 ± 7.8 mmHg (mean ± SD) dropped to 14.4 ± 3.4 mmHg at six months and 14.3 ± 3.1 mmHg at 12 months postoperatively (Figure 3), yielding average IOP reductions of 11.9 ± 7.4 mmHg (42.4 %) and 11.6 ± 8.4 mmHg (40.7 %), respectively (p<0.001). The patterns of IOP reduction were similar at all three surgical centres. Defining success as 5≤IOP≤18 and 20 % IOP reduction, the complete success rates after six and 12 months were 76.7 % (95 % CI 0.58–0.90) and 60 % (95 % CI 0.40–0.77), respectively, whereas qualified success rates were 83.3 % (95 % CI 0.65–0.94) and 86.6 % (95 % CI 0.69–0.96), respectively. Preoperative use of hypotensive medications per patient dropped from an average of 2.5 ± 1.3 to 0.1 ± 0.4 at six months and 0.6 ± 0.9 at 12 months (p<0.001). Eight needling procedures were performed in seven patients between one and four weeks (mean 3.8 weeks) after surgery. Two patients underwent YAG laser goniopuncture. One patient had it done two weeks after the initial surgery whereas the other was performed after four weeks.

Discussion

Manual NPDS achieves IOP reduction by facilitating outflow of aqueous humor through the thin trabeculo-Descemet’s membrane. The procedure is relatively safe but the surgical technique demands a high level of proficiency, with controversial efficacy relative to that of trabeculectomy.8,24
Theoretically, laser-assisted glaucoma surgery offers the potential advantage of improved accuracy, repeatability and safety. An increasing number of different radiation sources were examined for penetrating25–28 and non-penetrating22,25,29 glaucoma surgery with various success rates.
CO2 laser is commonly used in laser-assisted operations.30 Inherent characteristics of the CO2 laser make it suitable for a simplified non-penetrating filtration surgery, in particular its effective photoablation of dry tissues as well as its effective absorption by any amount of water or aqueous present. CLASS procedure uses the CO2 laser as a radiation source for gradual removal of scleral tissue layers, leaving the thin trabeculo-Descemet’s membrane through which aqueous percolates. The percolating fluid readily absorbs the laser’s energy, protecting the remaining tissue from further ablation and undesired perforation leaving the trabeculo-Descemet’s membrane intact.
The feasibility and safety of CLASS using the early prototype (OT-133) were studied in experimental models19 and in pilot clinical studies.11 The OT-133 was superseded by the design of the IOPtiMate system, implementing a revised beam-manipulating system, which was designed to improve and refine control of the ablation process and further decrease residual laser-induced thermal damage to the tissue.
IOPtiMate was proved to have a high safety profile in preclinical and clinical studies. No technical complications related to the intraoperative device were recorded. There was no intraoperative or postoperative evidence of damage to adjacent tissues (sclera, cornea, iris base, ciliary body). There were no cases of persistent hypotony during follow-up and no inflammatory reaction was recorded in the adjacent tissues, the AC or the vitreous cavity. No device-related macro-perforations were observed whereas micro-perforations occurred in both experimental and clinical models using the IOPtiMate system. Small holes that are not associated with loss of AC depth and/or iris prolapse may improve aqueous drainage and do not necessitate conversion to penetrating trabeculectomy.13 Some surgeons indeed advocate purposeful micro-puncturing of the canal roof with a needle to improve fluid flow. We do not recommend this practice, but at the same time we do not regard micro-perforation as harmful. Postoperative complications were not considered serious and were mostly mild and transitory.
The main drawback of using lasers for filtration procedures is the potential collateral damage induced by the scattered energy. Collateral thermal damage adjacent to the sclerostomy site is believed to be detrimental to the long-term success of the procedure. Using a laser with high water absorbance and low light scattering reduces the extent of collateral damage and improves the long-term success. Thermal damage is an integral effect of CO2 laser and was believed to be a major limiting factor in the success rate using the OT-133 laser system. The IOPtiMate system provides faster scanning, higher power focused laser beam, evenly distributed over the scanned area with some beam overlap to ensure uniform, effective ablation with minimal coagulative thermal damage to adjacent tissues. The energy is deposited using a scanner, which rapidly scans the focused laser beam across the treatment zone. The scanner is designed to move the focused beam such that the dwell time of the focused beam at each point is less than the thermal relaxation time, the characteristic heat conduction time constant in the tissue. Histological sections of experimental models showed minimal thermal damage at the lateral walls of the craters with no such damage at the bottom percolating zone, ensuring that the IOPtiMate system involves reduced heat damage.
The IOPtiMate system evaluated in laboratory models demonstrated precise scleral ablation with adequate percolation at the drainage area. The short- and intermediate-term efficacy of the CLASS procedure found in patients with open-angle and pseudoexfoliative glaucoma was at least comparable to that reported in a meta-analysis of the results of manual NPDS.31 The meta-analysis calculated a mean IOP≤21 mmHg at a mean follow-up of 31.3 months, achieved by 48.6 % of patients without any implant or antimetabolite medications, by 68.7 % of patients with an implant and by 67.1 % of patients upon use of antimetabolites.
The convenience of micro-dissecting under direct microscopic observation and the simplicity of performance are appealing advantages. CLASS obviates the prolonged learning curve characteristic of manual NPDS. The surgeon does not need to manually dissect layers of sclera or locate the orifice of the Schlemm’s canal, as in manual NPDS techniques. Instead, the surgeon gradually ablates an area which is easily identified by the use of simple landmarks (aiming dots of the scan pattern positioned on the surgical limbus). Once fluid is seen percolating, the natural drainage apparatus is clearly visible and the emerging fluid prevents further damage and perforation of the remaining thinned tissue.
Higher success rates may be achieved by augmenting the technique with implants or antimetabolites. Longer-term follow-up is required to evaluate the safety and long-term efficacy of CLASS, and to assess its usefulness in a wider spectrum of indications.
In summary, the use of laser technology to improve surgical accuracy is a highly appealing option. The feasibility and safety of CLASS using the IOPtiMate system were successfully demonstrated in all investigated models by the achievement of percolation with no evidence of device-related macro-perforation. ■

Article Information:
Disclosure

The studies were supported by a grant from IOPtima Ltd, Ramat-Gan, Israel.

Correspondence

Geffen Noa, Department of Ophthalmology, Meir Medical Center, 59 Tchernichovsky Street, 44281 Kfar Saba, Israel. E: noatal1122@gmail.com

Received

2011-09-20T00:00:00

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